MASP1 ELISA Kit (Mouse) (OKCD02714)

Catalog No: OKCD02714

Size:96WELLS

Mouse MASP1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-103%	91-105%	91-99%	83-94%
  EDTA Plasma (n=5)	89-99%	84-104%	84-97%	78-97%
  Heparin Plasma (n=5)	91-98%	88-95%	80-90%	96-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Associated Serine Protease 1 (MASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Associated Serine Protease 1 (MASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Associated Serine Protease 1 (MASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mannose Associated Serine Protease 1 (MASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-99	85
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma(n=5)	84-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Associated Serine Protease 1 (MASP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Associated Serine Protease 1 (MASP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MASP1 Microplate: 96 Wells (12 x 8 Well strips)
  • MASP1 Lyophilized Standard: 2
  • 100X Biotinylated MASP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 6.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mannose Associated Serine Protease 1 (MASP1). No significant cross-reactivity or interference between Mannose Associated Serine Protease 1 (MASP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Masp1
• Gene Full Name: mannan-binding lectin serine peptidase 1
• Alias Symbols: AW048060;CCPII;complement factor MASP-3;complement-activating component of Ra-reactive factor;Cr;Crarf;mannan-binding lectin serine protease 1;mannose-binding lectin-associated serine protease 1;mannose-binding protein-associated serine protease;MASP-1;Masp1/3;ra-reactive factor serine protease p100;raRF;serine protease 5.
• NCBI Gene Id: 17174
• Protein Name: Mannan-binding lectin serine protease 1
• Description of Target: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Also plays a role in development.
• Uniprot ID: P98064
• Protein Accession #: NP_032581.2
• Nucleotide Accession #: NM_008555.2
• Protein Size (# AA): 704