MARS Antibody - middle region (OAGA03483)

Catalog No: OAGA03483

Size:100 ul

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.

MARS antibody [N3C2], Internal detects MARS protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MARS protein stained by MARS antibody [N3C2], Internal diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MARS. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.67 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: MARS
• Gene Full Name: methionyl-tRNA synthetase
• Alias Symbols: MRS, ILLD, MARS, CMT2U, ILFS2, METRS, MTRNS, SPG70
• NCBI Gene Id: 4141
• Protein Name: methionine--tRNA ligase, cytoplasmic
• Description of Target: This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules.
• Uniprot ID: P56192
• Protein Accession #: NP_004981.2
• Nucleotide Accession #: NM_004990.3