MARK3 Antibody (OAAF02209)

Catalog No: OAAF02209 (Formerly GWB-ASD464)

Size:100 ug

Western blot analysis of extracts from HeLa/293 cells, using MARK3 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using MARK3 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MARK3.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MARK3 antibody detects endogenous levels of total MARK3 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:5000
• Reference: Peng C.Y., Cell Growth Differ. 9:197-208(1998).
  Sun T.-Q., Nat. Cell Biol. 3:628-636(2001).
  Wissing J., Mol. Cell. Proteomics 6:537-547(2007)

Target Info

• Gene Symbol: MARK3
• Gene Full Name: microtubule affinity regulating kinase 3
• Alias Symbols: cdc25C-associated protein kinase 1;CTAK1;C-TAK1;ELKL motif kinase 2;EMK-2;KP78;MAP/microtubule affinity-regulating kinase 3;Par-1a;PAR1A;protein kinase STK10;ser/Thr protein kinase PAR-1;serine/threonine-protein kinase p78;VIPB.
• NCBI Gene Id: 4140
• Protein Name: MAP/microtubule affinity-regulating kinase 3
• Description of Target: Serine/threonine-protein kinase (PubMed:23666762). Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed:16980613). Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed:28087714).
• Uniprot ID: P27448
• Protein Accession #: NP_001122390.1
• Molecular Weight: 86 kDa