MAPT ELISA Kit (Pig) (OKCA02515)

Catalog No: OKCA02515

Size:96 Wells

Pig MAPT ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Pig
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	96	92-100
  1:2	92	88-96
  1:4	84	80-88
  1:8	95	90-100

• ELISA Kit Principle: Aviva Systems Biology MAPT ELISA Kit (Pig) (OKCA02515) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAPT has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAPT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard MAPT captured in the well.
• ELISA Kit Range: 62.5 - 4,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	94	90-99
  EDTA Plasma (n=4)	89	84-93

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • MAPT Microplate: 96 Wells (12 x 8 Well strips)
  • MAPT Lyophilized Standard: 2
  • 100X Biotinylated MAPT Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MAPT
• Alias Symbols: tau
• NCBI Gene Id: 281296
• Protein Name: Microtubule-associated protein tau
• Description of Target: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
• Uniprot ID: P29172
• Protein Accession #: NP_776531
• Nucleotide Accession #: NM_174106