Size:96 Wells
$469.00
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SKU
OKEH01411
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MAPT ELISA Kit (Mouse) : 96 Wells (OKEH01411)

Catalog#: OKEH01411
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityMouse
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology MAPT ELISA Kit (Mouse) (OKEH01411) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAPT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAPT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAPT captured in well.
Kit Range15.6 - 1000pg/ml
Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.0% (n = 20)
Kit Component
ComponentAmount
MAPT Microplate96 Wells (12 x 8 Well strips)
MAPT Lyophilized Standard2
100X Biotinylated MAPT Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity8.14 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Mouse Microtubule-associated protein tau
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMAPT
Official Gene Full Namemicrotubule-associated protein tau
Alias SymbolsMicrotubule-associated protein tau, Mtapt, Tau, AI413597; AW045860
NCBI Gene Id17762
Protein NameMicrotubule-associated protein tau
Description of TargetPromotes microtubule assembly and stability and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Swissprot IdP10637
Protein Accession #NP_001033698.1
Nucleotide Accession #NM_001038609.2
Protein Size (# AA)733
Molecular Weight76 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express MAPT.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express MAPT.
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