MAPT Antibody (Phospho-Ser396) (OAAF00390)

Catalog No: OAAF00390 (Formerly GWB-ASB826)

Size:100 ug

Western blot analysis of extracts from HeLa cells, using Tau (Phospho-Ser396) Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Target Modification: Phospho
  Modification Sites:
  Human: Ser396
  Mouse: Ser688
  Rat: Ser707
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Tau around the phosphorylation site of Ser396.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Specificity: Tau (phospho-Ser396) antibody detects endogenous levels of Tau only when phosphorylated at serine 396.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~3000
  ELISA: 1:20000
• Reference: Mario Binelli, Biol Reprod, Feb 2001; 64: 654.
  Jens Leipziger, Am J Physiol Renal Physiol, Nov 2000; 279: 919.
  Yuuki Kawamura, Eur. J. Biochem., May 2001; 268: 3036.
  Xiaoying Koh, Biophys. J., Mar 2006; 90: 1999 - 2014.

Target Info

• Gene Symbol: MAPT
• Gene Full Name: microtubule associated protein tau
• Alias Symbols: DDPAC;FTDP-17;G protein beta1/gamma2 subunit-interacting factor 1;MAPTL;microtubule-associated protein tau;MSTD;MTBT1;MTBT2;neurofibrillary tangle protein;paired helical filament-tau;PHF-tau;PPND;PPP1R103;protein phosphatase 1, regulatory subunit 103;TAU;tau-40.
• NCBI Gene Id: 4137
• Protein Name: Microtubule-associated protein tau
• Description of Target: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
• Uniprot ID: P10636
• Protein Accession #: NP_001116538.2
• Molecular Weight: 78 kDa