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MAPT Antibody - middle region (ARP48103_P050)

100 ul
$289.00
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Conjugation Options

ARP48103_P050-FITC Conjugated

ARP48103_P050-HRP Conjugated

ARP48103_P050-Biotin Conjugated

Gene Symbol:
MAPT
Official Gene Full Name:
Microtubule-associated protein tau
NCBI Gene Id:
4137
Protein Name:
Microtubule-associated protein tau
Swissprot Id:
P10636-8
Protein Accession #:
NP_005901
Nucleotide Accession #:
NM_005910
Alias Symbols:
DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU
Replacement Item:
This antibody may replace item sc-166062 from Santa Cruz Biotechnology.
Description of Target:
MAPT is differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. The mutations in the gene have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Protein Size (# AA):
441
Molecular Weight:
46kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
IHC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MAPT.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MAPT.
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human MAPT
Predicted Species Reactivity:
Human, Mouse, Pig, Rat
Tested Species Reactivity:
Human, Mouse
Predicted Homology Based on Immunogen Sequence:
Human: 100%; Mouse: 86%; Pig: 79%; Rat: 86%
Complete computational species homology data:
Anti-MAPT (ARP48103_P050)
Peptide Sequence:
Synthetic peptide located within the following region: RGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPG
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
Ywhaz; Prkaca; STUB1; PHGK; SRC; PRKAR1A; GSK3B; FYN; DCTN1; CSNK1A1; CDK2; AKT1; ABL1; YWHAQ; SGK1; MARK2; MARCH7; TUBB3; TUBA1B; UBC; TUBA4A; MARK4; PEG10; MARK3; MARK1; MAPK13; MAPK11; MAPK8; mapk14; Mapk12; LIMS1; HSPA1A; PPP5C; PPP2R4; CAPN2; PKN1; H
Blocking Peptide:
For anti-MAPT (ARP48103_P050) antibody is Catalog # AAP48103 (Previous Catalog # AAPS21802)
Datasheets/Manuals:
Printable datasheet for anti-MAPT (ARP48103_P050) antibody
Target Reference:
Reynolds,M.R., (2006) Biochemistry 45 (13), 4314-4326

Product Reviews

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3 Item(s)

02/01/2017 16:23
  • Overall Experience:
  • Quality:
Product Review: MAPT antibody - middle region (ARP48103_P050) in E.Coli: expressed human Tau-C, Tau-E, Tau-F, Tau-B, Fetal-Tau, Tau-D using Western blot
Product Page for MAPT antibody - middle region (ARP48103_P050)

Researcher: Max Holzer, Paul Flechsig Institut of Brain Research, University of Leipzig
Application: Western blotting
Species + Tissue/Cell type:
Lane 1: 10ug E.Coli: expressed human Tau-C
Lane 2: 10ug E.Coli: expressed human Tau-E
Lane 3: 10ug E.Coli: expressed human Tau-F
Lane 4: 10ug E.Coli: expressed human Tau-B
Lane 5: 10ug E.Coli: expressed human Fetal-Tau
Lane 6: 10ug E.Coli: expressed human Tau-D
Primary antibody dilution: 1:1000
Secondary antibody: Donkey anti-rabbit-HRP
Secondary antibody dilution: 1:10,000


 Questionnaire:
 How do Aviva's reagents play a role in your experimental goals? Looking for a tau specific antiserum in IHC.
 How would you rate this antibody on a scale from 1-5 (5=best) nad why? 5
 Would you use this antibody in future experiment? Probably.
 Have you used another antibody which has worked in your application? Yes, ARP48103 perfomed very well.
 Do you believe the information about the reagent on Aviva's website is correct? Yes.
 If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? Probably.
 How did you store the antibody after re-suspension? Added glycerol to 40% final concentration, stored at -20C.
 Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): Recombinant human tau protein expressed in E.coli and N2A neuroblastoma cells.
 How many different experimental trials were conducted using the antibody sample? 3
 How was this sample prepared? Purified recombinant protein from E.coli or N2A cell lysate in Laemmli buffer was used.
 Primary antibody dilution and incubation time: 1:1000, overnight in blocking solution (TBST + 1% BSA) at 10C.
 Secondary antibody used and dilution and incubation time: Donkey anti-rabbit HRP 1:10000.
 What controls were used in your experiment (positive/negative)? Polyclonal tau antibody from DAKO serves as positive control.
 Please include your detailed WB Procedure/Protocol here: 10ug protein in standard reducing SDS-sample buffer (Laemmli) run on 8% SDS-PAGE, protein transfer overnight using tank blot and transfer buffer (48mM Tris, 39mM glycin, 20% methanol), blocking and dilution buffer TBS 0.1% Tween-20 with 1%BSA, wash buffer same as dilution buffer without BSA, primary antibody incubation overnight at 10C 1:1000 - 1:2000, secondary antibody 1:10000 60min at room temp., ECL development using Imaging station DNR Chembis 1.6.
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02/01/2017 16:23
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  • Quality:
Product Review: MAPT antibody - middle region (ARP48103_P050) in N2A expressed human Tau-C, Tau-E, Tau-F, Tau-B, Fetal-Tau, Tau-D, Fetal-Tau and N2A lysate using Western blot
Product Page for MAPT antibody - middle region (ARP48103_P050)

Researcher: Max Holzer, Paul Flechsig Institut of Brain Research, University of Leipzig
Application: Western blotting
Species + Tissue/Cell type:
Lane 1: 10ug N2A expressed human Tau-C
Lane 2: 10ug N2A expressed human Tau-E
Lane 3: 10ug N2A expressed human Tau-F
Lane 4: 10ug N2A expressed human Tau-B
Lane 5: 10ug N2A expressed human Fetal-Tau
Lane 6: 10ug N2A expressed human Tau-D
Lane 7: 10ug N2A expressed human much Fetal-Tau
Lane 8: 10ug N2A lysate
Primary antibody dilution: 1:2000
Secondary antibody: Donkey anti-rabbit-HRP
Secondary antibody dilution: 1:10,000


 Questionnaire:
 How do Aviva's reagents play a role in your experimental goals? Looking for a tau specific antiserum in IHC.
 How would you rate this antibody on a scale from 1-5 (5=best) nad why? 5
 Would you use this antibody in future experiment? Probably.
 Have you used another antibody which has worked in your application? Yes, ARP48103 perfomed very well.
 Do you believe the information about the reagent on Aviva's website is correct? Yes.
 If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? Probably.
 How did you store the antibody after re-suspension? Added glycerol to 40% final concentration, stored at -20C.
 Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): Recombinant human tau protein expressed in E.coli and N2A neuroblastoma cells.
 How many different experimental trials were conducted using the antibody sample? 3
 How was this sample prepared? Purified recombinant protein from E.coli or N2A cell lysate in Laemmli buffer was used.
 Primary antibody dilution and incubation time: 1:1000, overnight in blocking solution (TBST + 1% BSA) at 10C.
 Secondary antibody used and dilution and incubation time: Donkey anti-rabbit HRP 1:10000.
 What controls were used in your experiment (positive/negative)? Polyclonal tau antibody from DAKO serves as positive control.
 Please include your detailed WB Procedure/Protocol here: 10ug protein in standard reducing SDS-sample buffer (Laemmli) run on 8% SDS-PAGE, protein transfer overnight using tank blot and transfer buffer (48mM Tris, 39mM glycin, 20% methanol), blocking and dilution buffer TBS 0.1% Tween-20 with 1%BSA, wash buffer same as dilution buffer without BSA, primary antibody incubation overnight at 10C 1:1000 - 1:2000, secondary antibody 1:10000 60min at room temp., ECL development using Imaging station DNR Chembis 1.6.
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02/01/2017 16:23
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Product Review: MAPT antibody - middle region (ARP48103_P050) in Transgenic and WT mouse cortex using IHC
Product Page for MAPT antibody - middle region (ARP48103_P050)

Researcher: Max Holzer, Paul Flechsig Institut of Brain Research, University of Leipzig
Application: IHC
Species + Tissue/Cell type:
A: Transgenic hTau P301L mouse cortex
B: Tau-Ko mouse cortex
C: WT mouse cortex
Primary antibody dilution: 1:1000
Secondary antibody: Anti-rabbit-biotin / Avidin-HRP
Secondary antibody dilution: 1:1000 or 1:2000


Questionnaire:
How do Aviva's reagents play a role in your experimental goals? Looking for a tau specific antiserum in IHC.
How would you rate this antibody on a scale from 1-5 (5=best) and why? 5
Would you use this antibody in future experiments? Probably.
Have you used another antibody which has worked in your application? Yes, own monoclonal antibodies.
Do you believe the information about the reagent on Aviva's website is correct? Yes.
If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? Probably.
How did you store the antibody after re-suspension? Added glycerol to 40% final concentration, stored at -20C.
Sample Description (please include species type and tissue/cell type): Mouse brain tissue.
Please explain fixation solution/method used (formalin, periodate-lysine-paraformaldehyde, acetone, etc.)? Brain fixation by 4%PFA / 0.1% GA perfusion.
How many different experimental trials were conducted using the antibody sample? 3
Primary antibody dilution, incubation time and temperature: 1:1000 in TBS 0.1%Tween20, 1%BSA overnight at 10C, shaking platform.
Secondary antibody used, dilution, incubation time and temperature: Sheep anti-rabbit biotinylated secondary antibody 1:1000 2h room temp., Avidin-HRP conjugate 1:2000 1h room temp.
From your IHC/ICC images, briefly explain the colors of each stain and counterstain: Color development of staining was by diaminobenzidine with nickel enhancement, dark blue precipitate, no counterstain necessary.
Did you use an antigen retrieval method? If so, please explain? No.
What controls were used in your experiment? Ommision of primary antibody (negative control), use of validated own tau antibodies (positive control).
Please include your detailed tissue preparation and staining procedure/protocol here: Asphyxation animal with CO2,
perfusion animal with PBS/Heparin
perfusion animal with 4% PFA / 0.1% glutaraldehyde
preparation of brain from scull
postfixation in 4%PFA overnight
incubation in 30% sucrose for 48h
cutting 30um section with freezing microtome, free floating sections
blocking and dilution buffer TBS 0.1% Tween-20 with 1%BSA, wash buffer same as dilution buffer without BSA, primary antibody incubation overnight at 10C 1:1000, secondary antibody 1:1000 and  120min at room temp, Avidin-HRP 1:2000 60min room temp. color development with DAB/Ni
after staining fixing sections on slides and coverspliiping with entellan.
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