MAPKAPK5 Antibody - middle region (OAEB03098)

Catalog No: OAEB03098

Size:100UG

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IP, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: Mapkapk5
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat, Dog, Pig, Cow
• Peptide Sequence: C-STEALDNVLPSAQ
• Concentration: 0.5 mg/ml
• Epitope: middle region
• Application Info: P-ELISA: Antibody detection limit dilution 1:32000
  WB: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after OAEB03098 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1ug/ml.
  IHC: In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8ug/ml.
  IP: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug OAEB03098. Western blots of the IP were labelled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Target Info

• Gene Symbol: Mapkapk5|MAPKAPK5
• Gene Full Name: MAP kinase-activated protein kinase 5|MAPK activated protein kinase 5
• Alias Symbols: MAP kinase-activated protein kinase 5;MAPK-activated protein kinase 5;MAPKAP kinase 5;MAPKAPK-5;MAPKAP-K5;mitogen-activated protein kinase-activated protein kinase 5;MK5;MK-5;P;p38-regulated/activated protein kinase;PRAK.
• NCBI Gene Id: 17165|498183|8550
• Protein Accession #: NP_003659.2;
  NP_620777.1
• Molecular Weight: 54.0; 54.2