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Datasheets/Manuals | Printable datasheet for MAPKAPK2 Antibody - middle region (OAEB03100) |
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Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB, IP |
Additional Information | This antibody is expected to recognize both reported isoforms (NP_004750.1; NP_116584.2). |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | MAPKAPK2 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Peptide Sequence | C-SRVLKEDKERWED |
Concentration | 0.5 mg/ml |
Epitope | middle region (near C-terminal region) |
Application Info | Western Blot: Approx 60kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kDa according to Mouse NP_032577.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Menon et al, Cell Motil Cytoskeleton. 2009 Dec;66(12):1041-7. PMID: 19743408). There is no cross-reactivity to MK5. Peptide ELISA: antibody detection limit dilution 1:128000. Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2 (CST). An approx 45kDa of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. |
Gene Symbol | Mapkapk2|MAPKAPK2 |
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Gene Full Name | MAP kinase-activated protein kinase 2|MAPK activated protein kinase 2 |
Alias Symbols | AA960234;MAP kinase-activated protein kinase 2;MAPK-activated protein kinase 2;MAPKAP kinase 2;MAPKAP-K2;mitogen-activated protein kinase-activated protein kinase 2;MK2;MK-2;Rps6;Rps6kc1. |
NCBI Gene Id | 17164|289014|9261 |
Protein Accession # | NP_004750.1; NP_116584.2 |
Molecular Weight | 42.2; 45.6 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MAPKAPK2 Antibody - middle region (OAEB03100)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Rat".
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How long will it take to receive "MAPKAPK2 Antibody - middle region (OAEB03100)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "MAPKAPK2 Antibody - middle region (OAEB03100)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MAPKAPK2 Antibody - middle region (OAEB03100)"?
This target may also be called "AA960234;MAP kinase-activated protein kinase 2;MAPK-activated protein kinase 2;MAPKAP kinase 2;MAPKAP-K2;mitogen-activated protein kinase-activated protein kinase 2;MK2;MK-2;Rps6;Rps6kc1." in publications.
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What is the shipping cost for "MAPKAPK2 Antibody - middle region (OAEB03100)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MAPKAPK2 Antibody - middle region (OAEB03100)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MAPKAPK2 Antibody - middle region (OAEB03100)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "42.2; 45.6".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MAPKAPK2 Antibody - middle region (OAEB03100)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Mapkapk2|MAPKAPK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Mapkapk2|MAPKAPK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Mapkapk2|MAPKAPK2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Mapkapk2|MAPKAPK2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Mapkapk2|MAPKAPK2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Mapkapk2|MAPKAPK2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.