MAPK8IP1 ELISA Kit (Rat) (OKWB00352)

Catalog No: OKWB00352

Size:96T

Rat MAPK8IP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	95-99%	87-104%	90-101%	90-105%
  EDTA Plasma (n=5)	83-101%	94-101%	82-96%	87-100%
  Heparin Plasma (n=5)	80-98%	87-100%	80-97%	80-96%

• ELISA Kit Principle: Aviva Systems Biology Mapk8ip1 ELISA Kit (Rat) (OKWB00352) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mapk8ip1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mapk8ip1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mapk8ip1 captured in the well.
• ELISA Kit Range: 1.563-100ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	87-103	95
  EDTA Plasma (n=5)	86-102	93
  Heparin Plasma (n=5)	91-104	96

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Mapk8ip1 Microplate: 96 Wells (12 x 8 Well strips)
  • Mapk8ip1 Lyophilized Standard: 2 x 100 ng
  • 100X Mapk8ip1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.938 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mapk8ip1
• Gene Full Name: mitogen-activated protein kinase 8 interacting protein 1
• Alias Symbols: C-Jun-amino-terminal kinase-interacting protein 1;IB-1;islet-brain-1;Jip-1;JIP1;JIP-1-related protein;JNK MAP kinase scaffold protein 1;JNK-interacting protein 1;JRP;Mapk8ip;mitogen activated protein kinase 8 interacting protein;Mitogen-activated protein kinase 8-interacting protein 1.
• NCBI Gene Id: 116457
• Protein Name: C-Jun-amino-terminal kinase-interacting protein 1
• Description of Target: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and thus inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response (By similarity).
• Uniprot ID: Q9R237
• Protein Accession #: NP_446229
• Nucleotide Accession #: NM_053777