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Datasheets/Manuals | Printable datasheet for MAPK8 Antibody (Phospho-Thr183/TYR185) (OABF00788) |
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Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Porcine|Rabbit|Rat |
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Product Format | Liquid. Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot |
Additional Information | Subcellular location: Cytoplasm, Nucleus Modification: Phosphorylation (THR183, TYR185) |
Reconstitution and Storage | -20°C |
Immunogen | KLH conjugated synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183/Tyr185 |
Purification | Protein A Purified |
Concentration | 1 ug/ul |
Application Info | WB: 1:100 - 1000 IHC-P: 1:100 - 500 Fluorescent-IHC: 1:50 - 200 |
Gene Symbol | MAPK8 |
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Gene Full Name | mitogen-activated protein kinase 8 |
Alias Symbols | c-Jun N-terminal kinase 1;JNK;JNK1;JNK1A2;JNK21B1/2;JNK-46;JUN N-terminal kinase;MAP kinase 8;mitogen-activated protein kinase 8;PRKM8;SAPK1;SAPK1c;stress-activated protein kinase 1;stress-activated protein kinase 1c;Stress-activated protein kinase JNK1. |
NCBI Gene Id | 5599 |
Protein Name | Mitogen-activated protein kinase 8 |
Description of Target | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (By similarity). |
Uniprot ID | P45983 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MAPK8 Antibody (Phospho-Thr183/TYR185) (OABF00788)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Porcine|Rabbit|Rat".
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How long will it take to receive "MAPK8 Antibody (Phospho-Thr183/TYR185) (OABF00788)"?
This item is available "3 to 5 business days.".
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What buffer format is "MAPK8 Antibody (Phospho-Thr183/TYR185) (OABF00788)" provided in?
This item is provided in "Liquid. Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MAPK8 Antibody (Phospho-Thr183/TYR185) (OABF00788)"?
This target may also be called "c-Jun N-terminal kinase 1;JNK;JNK1;JNK1A2;JNK21B1/2;JNK-46;JUN N-terminal kinase;MAP kinase 8;mitogen-activated protein kinase 8;PRKM8;SAPK1;SAPK1c;stress-activated protein kinase 1;stress-activated protein kinase 1c;Stress-activated protein kinase JNK1." in publications.
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What is the shipping cost for "MAPK8 Antibody (Phospho-Thr183/TYR185) (OABF00788)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MAPK8 Antibody (Phospho-Thr183/TYR185) (OABF00788)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MAPK8 Antibody (Phospho-Thr183/TYR185) (OABF00788)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MAPK8 Antibody (Phospho-Thr183/TYR185) (OABF00788)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MAPK8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MAPK8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MAPK8"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MAPK8"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MAPK8"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MAPK8"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.