Human MAPK8 Antibody (Phospho-Thr183/Tyr185) (OAAQ00205)

Catalog No: OAAQ00205

Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or treated with K252a (red) or with UV+TPA (green) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 ug/mL. Cat. #OAAQ00205.

Peptide blocking flow cytometric analysis of 293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or with UV+TPA (green) or K252a and blocked with phospho-peptide (black) or UV and blocked with phospho pepeptide (gold) or K252a and blocked with non-phospho peptide (blue) or UV and blocked with non-phospho peptide (purple) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 ug/mL. Cat. #OAAQ00205.

Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or treated with K252a (red) or with UV+TPA (green) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 ug/mL. Cat. #OAAQ00205.

Peptide blocking flow cytometric analysis of 293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or with UV+TPA (green) or K252a and blocked with phospho-peptide (black) or UV and blocked with phospho pepeptide (gold) or K252a and blocked with non-phospho peptide (blue) or UV and blocked with non-phospho peptide (purple) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 ug/mL. Cat. #OAAQ00205.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA.
• Clonality: Monoclonal
• Clone: SAPKT183Y185-A11
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: Unconjugated
• Application: FC
• Reconstitution and Storage: -20C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human phospho SAPK/JNK
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Thr183/Tyr185
• Application Info: 1ug/mL - 0.001ug/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reference: Verheij M, Bose R, Lin XH, et al. (1996) Nature. 380: 75-79.
  Verheij M, Ruiter GA, Zerp SF, et al. (1998) Radiotherapy and Oncology. 47: 225-232.
  Moriguchi T, Toyoshima F, Masuyama N, et al. (1997) The EMBO Journal 16: 7045-7053.

Target Info

• Gene Symbol: MAPK8
• Gene Full Name: mitogen-activated protein kinase 8
• Alias Symbols: JNK, JNK1, PRKM8, SAPK1, JNK-46, JNK1A2, SAPK1c, JNK21B1/2
• NCBI Gene Id: 5599
• Protein Name: mitogen-activated protein kinase 8
• Description of Target: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Uniprot ID: P45983
• Protein Accession #: NP_001265476.1
• Nucleotide Accession #: NM_001278547.1