MAPK7 Antibody - C-terminal region (OAAB17061)

Catalog No: OAAB17061

Size:400ul

Western blot analysis of anti-ERK5 C-term Pab (Cat. # OAAB17061) in K562 cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody (C-term) (Cat.# OAAB17061), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: MAPK7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras- independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression
  Cellular Location: Cytoplasm. Nucleus. Note=Translocates to the nucleus upon activation
  Tissue Location: Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MAPK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 776-805 amino acids from the C-terminal region of human MAPK7.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: MAPK7
• Alias Symbols: BMK1, ERK4, ERK5, PRKM7
• NCBI Gene Id: 5598
• Protein Name: Mitogen-activated protein kinase 7
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q13164
• Protein Accession #: NP_002740.2
• Molecular Weight: 88386 Da