MAPK4 Antibody (OAGA00291)

Catalog No: OAGA00291

Size:100 ul

Sample(30 ug whole cell lysate)
A:H1299
7.5% SDS PAGE
OAGA00291 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded breast ca, using MAPK4(OAGA00291) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAPK4(OAGA00291) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus where it phosphorylates nuclear targets. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 326 of MAPK4 (Uniprot ID#P31152)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: MAPK4
• Gene Full Name: Mitogen-activated protein kinase 4
• Alias Symbols: ERK4, ERK-4, PRKM4, p63MAPK, p63-MAPK
• NCBI Gene Id: 5596
• Protein Name: Mitogen-activated protein kinase 4
• Uniprot ID: P31152
• Protein Accession #: NP_002738.2
• Molecular Weight: 66 kDa