Catalog No: OKEH04112
Size:96T
Price: $475.00
SKU
OKEH04112
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MAPK3 ELISA Kit (Human) (OKEH04112) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAPK3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAPK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAPK3 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.3% (n = 20)
Mean Inter-assay CV%: <= 6.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MAPK3 Microplate96 Wells (12 x 8 Well Strips)
MAPK3 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
MAPK3 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.067 ng/mL
SpecificityNatural and recombinant Human Mitogen-activated protein kinase 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMAPK3
Gene Full Namemitogen-activated protein kinase 3
Alias SymbolsERK1;ERK-1;ERT2;extracellular signal-regulated kinase 1;extracellular signal-related kinase 1;HS44KDAP;HUMKER1A;insulin-stimulated MAP2 kinase;MAP kinase isoform p44;MAPK 1;microtubule-associated protein 2 kinase;mitogen-activated protein kinase 3;p44-ERK1;P44ERK1;p44-MAPK;P44MAPK;PRKM3.
NCBI Gene Id5595
Protein NameP27361
Description of TargetMitogen-activated protein kinase 3
Uniprot IDhttps://www.uniprot.org/uniprot/P27361
Protein Accession #NP_001035145.1
Nucleotide Accession #NM_001040056.2
Protein Size (# AA)379
Molecular Weight43 kDa
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