Mapk3 Antibody (OASA07240)

Gene Symbol:: Mapk3
NCBI Gene Id:: 50689
Protein Name:: Mitogen-activated protein kinase 3
Swissprot Id:: P21708
Protein Accession #:: NP_059043.1
Alias Symbols:: p44, ERK1, ERT2, MNK1, Erk-1, Esrk1, MAPK1, Prkm3, p44erk1, p44mapk, Mapk3
Replacement Item:: This antibody may replace item sc-135900 from Santa Cruz Biotechnology.
Description of Target:: RABBIT ANTI ERK/MAPK (pThr202/pTyr204)
Protein Size (# AA):: 380
Host:: Rabbit
Clonality:: Polyclonal
Application:: WB
Tissue Tool:: Find tissues and cell lines supported by DNA array analysis to express Mapk3.
RNA Seq:: Find tissues and cell lines supported by RNA-seq analysis to express Mapk3.
Immunogen:: The immunogen for anti-Mapk3 antibody: synthetic phosphopeptide corresponding to an amino acid sequence within rat ERK/MAPK which includes phosphorylated Thr202 and Tyr204.
Species Reactivity:: Rat
Predicted Homology Based on Immunogen Sequence:: Rat
Product Format:: Purified IgG - liquid
Reconstitution and Storage:: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:: Printable datasheet for anti-Mapk3 antibody - OASA07240
Specificity:: ERK/MAPK

Isotype:: Polyclonal IgG
Application Info:: Western Blot: This product recognises a band of approximately 43kD in human T47D lysates.
* For the detection of phosphoproteins, threonine and tyrosine phosphatase inhibitors such as 10mM Sodium Fluoride and 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
:::: Preservative Stabilisers: 0.09% - Sodium Azide
0.01% - Bovine Serum Albumin
50% - Glycerol
Antiserum Preparation: Antisera to phosphorylated rat ERK/MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
:::: Buffer Solutions: 10mM HEPES pH7.5

Name	# of Products
arachidonic acid metabolic process	6
cell cycle	349
MAPK import into nucleus	4
positive regulation of transcription, DNA-dependent	694
positive regulation of translation	71
protein complex assembly	152
response to toxin	60

Name	# of Products
apoptotic process	586
arachidonic acid metabolic process	12
cartilage development	76
caveolin-mediated endocytosis	5
cell cycle	355
cellular response to mechanical stimulus	62
interleukin-1-mediated signaling pathway	19
intracellular protein kinase cascade	91
intracellular signal transduction	200
lipopolysaccharide-mediated signaling pathway	48
MAPK cascade	105
MAPK import into nucleus	7
negative regulation of apolipoprotein binding	3
organ morphogenesis	132
positive regulation of histone acetylation	21
positive regulation of histone phosphorylation	7
positive regulation of transcription from RNA polymerase II promoter	968
positive regulation of transcription, DNA-dependent	681
positive regulation of translation	41
protein complex assembly	100
protein phosphorylation	451
regulation of cytoskeleton organization	7
regulation of early endosome to late endosome transport	5
regulation of Golgi inheritance	5
regulation of sequence-specific DNA binding transcription factor activity	79
regulation of stress-activated MAPK cascade	7
response to DNA damage stimulus	144
response to exogenous dsRNA	14
response to lipopolysaccharide	175
response to toxin	75
sensory perception of pain	53
signal transduction by phosphorylation	12
transcription, DNA-dependent	402

Name	# of Products
ATP binding	2814
MAP kinase activity	71
nucleotide binding	3928
phosphatase binding	87
phosphotyrosine binding	59
protein binding	12191
protein kinase activity	636
protein serine/threonine kinase activity	738