Mapk3 Antibody (OASA07240)

Catalog#: OASA07240

• Size: 0.1 ml

• Predicted Species Reactivity: Rat
• Product Format: Purified IgG - liquid
• Clonality: Polyclonal
• Isotype: Polyclonal IgG
• Host: Rabbit
• Application: WB
  Preservative Stabilisers: 0.09% - Sodium Azide
  0.01% - Bovine Serum Albumin
  50% - Glycerol
  Antiserum Preparation: Antisera to phosphorylated rat ERK/MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
  Buffer Solutions: 10mM HEPES pH7.5
• Reconstitution and Storage: Store at +4^oC or at -20^oC if preferred.
  Storage in frost-free freezers is not recommended.
  This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
  
• Gene Symbol: Mapk3
• NCBI Gene Id: 50689
• Protein Name: Mitogen-activated protein kinase 3
• Swissprot Id: P21708
• Protein Accession #: NP_059043.1
• Alias Symbols: p44, ERK1, ERT2, MNK1, Erk-1, Esrk1, MAPK1, Prkm3, p44erk1, p44mapk, Mapk3
• Replacement Item: This antibody may replace item sc-135900 from Santa Cruz Biotechnology.
• Description of Target: RABBIT ANTI ERK/MAPK (pThr202/pTyr204)
  
• Protein Size (# AA): 380
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express Mapk3.
• Immunogen: The immunogen for anti-Mapk3 antibody: synthetic phosphopeptide corresponding to an amino acid sequence within rat ERK/MAPK which includes phosphorylated Thr202 and Tyr204.
• Predicted Homology Based on Immunogen Sequence: Rat
• Specificity: ERK/MAPK
• Application Info: Western Blot: This product recognises a band of approximately 43kD in human T47D lysates.
  * For the detection of phosphoproteins, threonine and tyrosine phosphatase inhibitors such as 10mM Sodium Fluoride and 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Protocol:

Tips Information: