Human MAPK14 Antibody (Phospho-Thr180/Tyr182) (OAAQ00020)

Catalog No: OAAQ00020

Flow cytometric analysis of A431 cells secondary antibody only (blue) or untreated with 0.01 ug/mL of isotype control Cat. #OAAQ00101 (orange) or untreated (red) or staurosporine-treated (green) using 0.01 ug/mL of Phospho-p38 MAPK (Thr180/Tyr182) antibody P38T180Y182-E3 Cat. #OAAQ00020.

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 ug/mL of isotype control Cat. #OAAQ00101 (orange) or untreated (red) or treated with staurosporine (green) using Phospho-p38 MAPK (Thr180/Tyr182) antibody P38T180Y182-E3 at 0.1 ug/mL. Cat. #OAAQ00020.

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using phospho-p38 MAPK (Thr180/Tyr182) antibody P38T180Y182-E3 0.01 ug/mL. Cat. #OAAQ00020.

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-p38 MAPK (Thr180/Tyr182) antibody P38T180Y182-E3 at 5ng/mL. Cat. #OAAQ00020.

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using phospho-p38 MAPK (Thr180/Tyr182) antibody P38T180Y182-E3 0.01 ug/mL. Cat. #OAAQ00020.

Flow cytometric analysis of A431 cells secondary antibody only (blue) or untreated with 0.01 ug/mL of isotype control Cat. #OAAQ00101 (orange) or untreated (red) or staurosporine-treated (green) using 0.01 ug/mL of Phospho-p38 MAPK (Thr180/Tyr182) antibody P38T180Y182-E3 Cat. #OAAQ00020.

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-p38 MAPK (Thr180/Tyr182) antibody P38T180Y182-E3 at 5ng/mL. Cat. #OAAQ00020.

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 ug/mL of isotype control Cat. #OAAQ00101 (orange) or untreated (red) or treated with staurosporine (green) using Phospho-p38 MAPK (Thr180/Tyr182) antibody P38T180Y182-E3 at 0.1 ug/mL. Cat. #OAAQ00020.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA.
• Clonality: Monoclonal
• Clone: P38T180Y182-E3
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: Unconjugated
• Application: FC
• Reconstitution and Storage: -20C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr180/Tyr182 of human phospho p38 MAPK.
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Thr180/Tyr182
• Application Info: 1ug/mL - 0.001ug/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reference: (1) Corre I, Paris F, Huot J. (2017) Oncotarget. 8:55684-55714.
  (2) Tudor C, Marchese FP, Hitti E, et al. (2009) FEBS Letters. 583: 1933-1938.

Target Info

• Gene Symbol: MAPK14
• Gene Full Name: mitogen-activated protein kinase 14
• Alias Symbols: RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA
• NCBI Gene Id: 1432
• Protein Name: mitogen-activated protein kinase 14
• Description of Target: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
• Uniprot ID: Q16539
• Protein Accession #: NP_001306.1
• Nucleotide Accession #: NM_001315.2