MAPK14 Antibody (OAED00105)

Catalog No: OAED00105

Size:100 ug

Western Blot analysis of p38 MAPK: Lane 1: MW Marker; Lane 2: HeLa, (Cell Lysate); Lane 3: Brain (mouse), (tissue extract); Lane 4: Brain (rat), (tissue extract).

IHC Analysis: Human placenta tissue stained with p38 MAPK, pAb at 10 ug/ml.

IHC Analysis: Human spleen tissue stained with p38 MAPK, pAb at 10 ug/ml.

IHC Analysis: Human tonsil tissue stained with p38 MAPK, pAb at 10 ug/ml.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, IP, WB
• Reconstitution and Storage: Long Term Storage : -20C
  
• Immunogen: Synthetic peptide corresponding to a portion of human p38. The sequence is completely conserved in mouse.
• Specificity: Recognizes human, mouse, rat, bovine, dog, chicken, guinea pig, hamster, monkey, pig, rabbit and sheep p38. Detects a band of ~38kDa by Western blot.
• Formulation: Liquid. Whole rabbit serum.
• Application Info: Immunohistochemistry (paraffin sections)
  Immunoprecipitation (1:500)
  Western Blot (1:10,000, ECL)
  Optimal conditions must be determined individually for each application.

Target Info

• Gene Symbol: MAPK14
• Gene Full Name: Mitogen-activated protein kinase 14
• Alias Symbols: RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA
• NCBI Gene Id: 1432
• Protein Name: Mitogen-activated protein kinase 14
• Description of Target: Six proteins of the p38 subfamily of MAP kinases have been identified to date, including: p38alpha (SAPK2, CSBP) and its splice variant Mxi; p38beta and beta2; p38gamma (ERK6, SAPK3); and p38delta (SAPK4). p38 MAP kinases contain a conserved TGY motif in their activation loop and are activated in response to cytokines, hormones, GPCRs, and osmotic and heat shock to mediate primarily pro-apoptotic and pro-inflammatory responses. MKK3, MKK4, and MKK6 are known to activate p38 family members with varying specificity. All four p38 isoforms are able to phosphorylate the activating transcription factor 2 (ATF2), and differentially activate numerous substrates including Mnk1/2, Msk, phospholipase A2, and the MAPKAP kinase-2 and -3. Activated MAPKAP kinase-2 subsequently phosphorylates the heat shock protein Hsp27, an F-actin binding protein involved in cytoskeletal maintenance.
• Uniprot ID: Q16539
• Protein Accession #: NP_001306.1; NP_620581.1; NP_620582.1; NP_620583.1
• Nucleotide Accession #: NM_001315.2; NM_139012.2; NM_139013.2; NM_139014.2