MAPK1 Antibody (OABB00269)

Catalog No: OABB00269

Size:100UG

Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Plancenta Tissue Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: Hela Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: Raji Cell Lysate
Lane 8: Colo320 Cell Lysate

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene. The gene is mapped to 22q11.2. And The protein encoded by this gene is a member of the MAP kinase family. The deduced 360-amino acid human ERK2 protein shares 98% identity with rat Erk2. The MAP kinase ERK2 is widely involved in eukaryotic signal transduction. Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets. Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. And the MAPK pathway is also involved in responses to NTN1.
  Background: Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene.1 The gene is mapped to 22q11.2.2 And The protein encoded by this gene is a member of the MAP kinase family. The deduced 360-amino acid human ERK2 protein shares 98% identity with rat Erk2.3 The MAP kinase ERK2 is widely involved in eukaryotic signal transduction. Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets.4 Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex.5 And the MAPK pathway is also involved in responses to NTN1.6
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
• Reference: 1. Owaki H, Makar R, Boulton TG, Cobb MH, Geppert TD (February 1992). "Extracellular
  signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs". Biochem.
  Biophys. Res. Commun. 182 (3): 1416–22.
  2. Li, L.; Wysk, M.; Gonzalez, F. A.; Davis, R. J. : Genomic loci of human mitogen-activated protein
  kinases. Oncogene 9: 647-649, 1994.
  3. Owaki, H.; Makar, R.; Boulton, T. G.; Cobb, M. H.; Geppert, T. D. : Extracellular signal-regulated
  kinases in T cells: characterization of human ERK1 and ERK2 cDNAs. Biochem. Biophys. Res.
  Commun. 182: 1416-1422, 1992.
  4. Khokhlatchev, A. V.; Canagarajah, B.; Wilsbacher, J.; Robinson, M.; Atkinson, M.; Goldsmith, E.;
  Cobb, M. H. : Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear
  translocation. Cell 93: 605-615, 1998.
  5. Di Cristo, G.; Berardi, N.; Cancedda, L.; Pizzorusso, T.; Putignano, E.; Ratto, G. M.; Maffei, L. :
  Requirement of ERK activation for visual cortical plasticity. Science 292: 2337-2340, 2001.
  6. Forcet, C.; Stein, E.; Pays, L.; Corset, V.; Llambi, F.; Tessier-Lavigne, M.; Mehlen, P. :
  Netrin-1-mediated axon outgrowth requires deleted in colorectal cancer-dependent MAPK activation.
  Nature 417: 443-447, 2002
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 1(MAPK1) detection. Tested with WB in Human;Mouse;Rat.

Target Info

• Gene Symbol: MAPK1
• Gene Full Name: mitogen-activated protein kinase 1
• Alias Symbols: ERK;ERK2;ERK-2;ERT1;extracellular signal-regulated kinase 2;MAP kinase 1;MAP kinase 2;MAP kinase isoform p42;MAPK 2;MAPK2;mitogen-activated protein kinase 1;mitogen-activated protein kinase 2;NS13;p38;p40;p41;p41mapk;p42-MAPK;P42MAPK;PRKM1;PRKM2;protein tyrosine kinase ERK2.
• NCBI Gene Id: 5594
• Protein Name: Mitogen-activated protein kinase 1
• Description of Target: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity).
• Uniprot ID: P28482
• Protein Accession #: NP_002736.3; NP_620407.1
• Nucleotide Accession #: NM_002745.4; NM_138957.3
• Molecular Weight: 41390 MW