MAP4K2 Antibody - middle region (OAAB16806)

Catalog No: OAAB16806

Size:400ul

The anti-MAP4K2 Pab (Cat. # OAAB16806) is used in Western blot to detect MAP4K2 in mouse skeletal tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: Germinal Center Kinase (GCK/MAP4K2) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3 May play a role in the regulation of vesicle targeting or fusion regulation of vesicle targeting or fusion
  Cellular Location: Cytoplasm (By similarity). Basolateral cell membrane; Peripheral membrane protein (By similarity). Golgi apparatus membrane; Peripheral membrane protein (By similarity)
  Tissue Location: Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Germinal Center Kinase (GCK/MAP4K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-319 amino acids from the Central region of human Germinal Center Kinase (GCK/MAP4K2).
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: MAP4K2
• Alias Symbols: GCK, BL44, RAB8IP
• NCBI Gene Id: 5871
• Protein Name: Mitogen-activated protein kinase kinase kinase kinase 2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q12851
• Protein Accession #: NP_004570.2
• Molecular Weight: 91556 Da