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Datasheets/Manuals | Printable datasheet for anti-MAP3K5 (ARP87397_P050) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human MAP3K5 |
Purification | Affinity purified |
Peptide Sequence | Synthetic peptide located within the following region: IRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQ |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-MAP3K5 (ARP87397_P050) antibody is Catalog # AAP87397 |
Gene Symbol | MAP3K5 |
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Gene Full Name | mitogen-activated protein kinase kinase kinase 5 |
Alias Symbols | ASK1, MEKK5, MAPKKK5 |
NCBI Gene Id | 4217 |
Protein Name | mitogen-activated protein kinase kinase kinase 5 |
Description of Target | Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. |
Uniprot ID | Q99683-2 |
Protein Accession # | NP_005914.1 |
Nucleotide Accession # | NM_005923.3 |
Protein Size (# AA) | 621 |
Molecular Weight | 68 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MAP3K5 Antibody - middle region (ARP87397_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "MAP3K5 Antibody - middle region (ARP87397_P050)"?
This item is available "Domestic: within 24 hours delivery | International: 3-5 days".
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What buffer format is "MAP3K5 Antibody - middle region (ARP87397_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MAP3K5 Antibody - middle region (ARP87397_P050)"?
This target may also be called "ASK1, MEKK5, MAPKKK5" in publications.
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What is the shipping cost for "MAP3K5 Antibody - middle region (ARP87397_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MAP3K5 Antibody - middle region (ARP87397_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MAP3K5 Antibody - middle region (ARP87397_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "68 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MAP3K5 Antibody - middle region (ARP87397_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MAP3K5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MAP3K5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MAP3K5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MAP3K5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MAP3K5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MAP3K5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.