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Catalog No: OKWB00142
Size:96T
Price: $450.00
SKU
OKWB00142
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for MAP34-A ELISA Kit (Goat) (OKWB00142)
Product Info
Predicted Species ReactivityCapra hircus|Goat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-105%88-98%92-104%86-104%
EDTA Plasma (n=5)85-99%88-100%84-95%87-97%
Heparin Plasma (n=5)80-94%90-102%83-98%84-99%
ELISA Kit PrincipleAviva Systems Biology Map34-a ELISA Kit (Goat) (OKWB00142) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Map34-a has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Map34-a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Map34-a captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)89-10597
EDTA Plasma (n=5)91-9994
Heparin Plasma (n=5)86-10595
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Map34-a Microplate96 Wells (12 x 8 Well strips)
Map34-a Lyophilized Standard2 x 10 ng
100X Map34-a Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.094 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolMAP34-B
Gene Full NameMAP34-B protein
Alias Symbolsmap34-A;MAP34-B protein.
NCBI Gene Id100861275
Protein NameMAP34-A protein
Uniprot IDP82017
Protein Accession #NP_001272549
Nucleotide Accession #NM_001285620
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