MAP2K7 Antibody (Phospho-Thr275) (OAAB20469)

Catalog No: OAAB20469

Size:100ul

Western blot analysis of extracts from cos-7 cells (Lane 2) and 3T3 cells (Lane 3), using MAP2K7 (Phospho-Thr275) Antibody (OAAB20469). The lane on the left is treated with synthesized peptide.

Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MAP2K7 (Phospho-Thr275) antibody (OAAB20469).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB
• Additional Information: Function: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Target Post-Translational Modification: Phospho-Thr275
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:1000
• Reference: Wu Z.,et al.Mol. Cell. Biol. 17:7407-7416(1997).
  Lu X.,et al.J. Biol. Chem. 272:24751-24754(1997).
  Foltz I.N.,et al.J. Biol. Chem. 273:9344-9351(1998).
  Michael L.,et al.Biochem. Biophys. Res. Commun. 341:679-683(2006).
  Yang J.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
  

Target Info

• Gene Symbol: MAP2K7
• Gene Full Name: mitogen-activated protein kinase kinase 7
• Alias Symbols: MEK, MKK7, JNKK2, MEK 7, MAPKK7, PRKMK7, SAPKK4, SAPKK-4
• NCBI Gene Id: 5609
• Description of Target: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants.
• Uniprot ID: O14733
• Protein Accession #: NP_001284484.1
• Nucleotide Accession #: NM_001297555.1
• Molecular Weight: 47 kDa