MAP2K7 Antibody (OACD05421)

Catalog No: OACD05421

Western Blot with Mouse Recombinant Dual specificity mitogen-activated protein kinase kinase 7

Western Blot with Mouse Recombinant Dual specificity mitogen-activated protein kinase kinase 7

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Range: Met181 - His429
• Purification: Affinity Purified; Protein A Purified
• Concentration: 0.5 mg/ml
• Preservative: 0.02% NaN3
• Specificity: The antibody is a rabbit polyclonal antibody raised against MAP2K7. It has been selected for its ability to recognize MAP2K7 in immunohistochemical staining and western blotting.
• Formulation: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol
• Application Data: Western blotting: 1:50-400
  Immunocytochemistry in formalin fixed cells: 1:50-500
  Immunohistochemistry in formalin fixed frozen section: 1:50-500
  Immunohistochemistry in paraffin section: 1:10-100
  Enzyme-linked Immunosorbent Assay: 1:100-1:5,000
  Optimal working dilutions must be determined by end user.

Target Info

• Gene Symbol: Map2k7
• Gene Full Name: mitogen-activated protein kinase kinase 7
• Alias Symbols: 5930412N11Rik;c-Jun N-terminal kinase kinase 2;dual specificity mitogen-activated protein kinase kinase 7;JNK kinase 2;JNK-activating kinase 2;Jnkk;JNKK 2;Jnkk2;MAP kinase kinase 7;MAPK/ERK kinase 7;MAPKK 7;Mapkk7;MEK 7;Mek7;Mkk7;Prkm;Prkmk7;protein kinase, mitogen activated, kinase 7;sek;sek2.
• NCBI Gene Id: 26400
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• Description of Target: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
• Uniprot ID: Q8CE90
• Protein Accession #: NP_001036022.1
• Nucleotide Accession #: NM_001042557
• Molecular Weight: 39 kDa