MAP2K3 Antibody (Phospho-Thr222) (OAAF00457)

Catalog No: OAAF00457 (Formerly GWB-ASB900)

Size:100 ug

Western blot analysis of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MAP2K3 around the phosphorylation site of Thr222.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MAP2K3 (Phospho-Thr222) antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:1000
• Reference: Derijard B., Science 267:682-685(1995).
  Moriguchi T., J. Biol. Chem. 271:26981-26988(1996).
  The MGC Project Team; Genome Res. 14:2121-2127(2004).

Target Info

• Gene Symbol: MAP2K3
• Gene Full Name: mitogen-activated protein kinase kinase 3
• Alias Symbols: dual specificity mitogen-activated protein kinase kinase 3;MAP kinase kinase 3;MAPK/ERK kinase 3;MAPKK 3;MAPKK3;MEK 3;MEK3;MKK3;PRKMK3;SAPK kinase 2;SAPKK2;SAPKK-2;stress-activated protein kinase kinase 2.
• NCBI Gene Id: 5606
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 3
• Description of Target: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
• Uniprot ID: P46734
• Protein Accession #: NP_002747.2
• Molecular Weight: 39 kDa