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Datasheets/Manuals | Printable datasheet for anti-MAP2K3 (OASG04479) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | WB, IHC |
Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
Immunogen | Synthesized peptide derived from human MEK-3 around the phosphorylation site of S189. Location: 150-230aa. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phospho-Ser218 |
Specificity | Phospho-MEK-3 (S189) Polyclonal Antibody detects endogenous levels of MEK-3 protein only when phosphorylated at S189. |
Application Info | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; |
Gene Symbol | MAP2K3 |
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Gene Full Name | mitogen-activated protein kinase kinase 3 |
Alias Symbols | MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2 |
NCBI Gene Id | 5606; 26397; |
Description of Target | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. |
Uniprot ID | P46734, O09110 |
Molecular Weight | 39 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MAP2K3 Antibody (Phospho-Ser218) (OASG04479)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "MAP2K3 Antibody (Phospho-Ser218) (OASG04479)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
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What buffer format is "MAP2K3 Antibody (Phospho-Ser218) (OASG04479)" provided in?
This item is provided in "Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MAP2K3 Antibody (Phospho-Ser218) (OASG04479)"?
This target may also be called "MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2" in publications.
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What is the shipping cost for "MAP2K3 Antibody (Phospho-Ser218) (OASG04479)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MAP2K3 Antibody (Phospho-Ser218) (OASG04479)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MAP2K3 Antibody (Phospho-Ser218) (OASG04479)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "39 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MAP2K3 Antibody (Phospho-Ser218) (OASG04479)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MAP2K3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MAP2K3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MAP2K3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MAP2K3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MAP2K3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MAP2K3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.