MAP2K3 Antibody (OAAF01373)

Catalog No: OAAF01373

Size:100 ug

Western blot analysis of extracts from MDA-MB-435 cells, using MKK6 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MKK6 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MKK6.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MKK6 (Ab-207)Antibody detects endogenous levels of total MKK6 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:5000
• Reference: Wang W, et al. (2002)Mol Cell Biol ; 22(10): 3389-403.
  Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

Target Info

• Gene Symbol: MAP2K6
• Gene Full Name: mitogen-activated protein kinase kinase 6
• Alias Symbols: dual specificity mitogen-activated protein kinase kinase 6;MAPK/ERK kinase 6;MAPKK 6;MAPKK6;MEK 6;MEK6;MKK6;PRKMK6;protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6);SAPK kinase 3;SAPKK3;SAPKK-3;stress-activated protein kinase kinase 3.
• NCBI Gene Id: 5608
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 6
• Description of Target: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
• Uniprot ID: P52564
• Protein Accession #: NP_002747.2
• Molecular Weight: 37 kDa