Human MAP2K1/MAP2K2 Antibody : APC (Phospho-Ser217/221) (OAAQ00183)

Catalog No: OAAQ00183

Flow cytometric analysis of Hela cells unstained treated with imatinib as negative control (blue) or stained treated with imatinib (red) or treated with pervanadate (green) using Phospho-MEK1/2 (S217/221) antibody MEK12S217S221-H2 APC conjugate, Cat#OAAQ00183.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.09% NaN3, 0.2% BSA.
• Clonality: Monoclonal
• Clone: MEK12S217S221-H2
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: APC: Allophycocyanin
• Application: FC
• Reconstitution and Storage: 2-8C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser217/221 of human phospho MEK1/2.
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Ser217/221
• Application Info: For flow cytometric staining, the suggested use of this reagent is 5 uL per million cells or 5 uL per 100 uL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
• Reference: Raman M. and Cobb MH., (2003) Curr Biol 13:R886-888.
  Kuida K., and Boucher DM. (2004) J Biochem 135:653-656.
  Torii S., et al., (2004) J Biochem, 136:557-561.
  Yoon S. and Seger R., (2006) Growth Factors 24:21-44.
  Natel A. et al., (1998) J Biol Chem 273:10475-10484.

Target Info

• Gene Symbol: MAP2K1, MAP2K2
• Gene Full Name: mitogen-activated protein kinase kinase 1, mitogen-activated protein kinase kinase 2
• Alias Symbols: MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1
• NCBI Gene Id: 5604; 5605
• Protein Name: dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2
• Description of Target: The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
  The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.
• Uniprot ID: Q02750, P36507
• Protein Accession #: NP_002746.1
• Nucleotide Accession #: NM_002755.3