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MAP2K1 Antibody (OASA08354)

0.1ml
$770.00
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Gene Symbol:
MAP2K1
NCBI Gene Id:
5604
Protein Name:
Dual specificity mitogen-activated protein kinase kinase 1
Swissprot Id:
Q02750
Protein Accession #:
NP_002746.1
Alias Symbols:
MEK1, MKK1, MAPKK1, PRKMK1, MAP2K1
Replacement Item:
This antibody may replace item sc-436 from Santa Cruz Biotechnology.
Description of Target:
RABBIT ANTI MEK 1 (pThr386)
Protein Size (# AA):
393
Host:
Rabbit
Clonality:
Polyclonal
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MAP2K1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MAP2K1.
Immunogen:
The immunogen for anti-MAP2K1 antibody: synthetic phosphopeptide corresponding to an amino acid sequence within human MEK 1 which includes phosphorylated Thr386.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-MAP2K1 antibody - OASA08354
Specificity:
MEK 1
Isotype:
Polyclonal IgG
Application Info:
Western Blot: This product detects a band of approximately 45kDa in human T47D lysates .
* For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
:::
Preservative Stabilisers: 0.09% - Sodium Azide
0.01% - Bovine Serum Albumin
50% - Glycerol
Antiserum Preparation: Antisera to phosphorylated MEK 1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
:::
Buffer Solutions: 10mM HEPES pH7.5

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