Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH01676
Size:96T
Price: $600.00
SKU
OKEH01676
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

MAP1LC3B ELISA Kit (Human) : 96 Wells (OKEH01676)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MAP1LC3B ELISA Kit (Human) (OKEH01676) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAP1LC3B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAP1LC3B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAP1LC3B captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
MAP1LC3B Microplate96 Wells (12 x 8 Well strips)
MAP1LC3B Lyophilized Standard2
100X Biotinylated MAP1LC3B Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Microtubule-associated proteins 1A/1B light chain 3B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMAP1LC3B
Gene Full Namemicrotubule associated protein 1 light chain 3 beta
Alias SymbolsATG8F;Autophagy-related protein LC3 B;autophagy-related ubiquitin-like modifier LC3 B;LC3B;MAP1 light chain 3-like protein 2;MAP1A/1BLC3;MAP1A/MAP1B LC3 B;MAP1A/MAP1B light chain 3 B;MAP1LC3B-a;Microtubule-associated protein 1 light chain 3 beta;microtubule-associated proteins 1A/1B light chain 3B.
NCBI Gene Id81631
Protein NameQ9GZQ8
Description of TargetMicrotubule-associated proteins 1A/1B light chain 3B
Uniprot IDhttps://www.uniprot.org/uniprot/Q9GZQ8
Protein Accession #NP_073729.1
Nucleotide Accession #NM_022818.4
Protein Size (# AA)125
Molecular Weight15 kDa
Write Your Own Review
You're reviewing:MAP1LC3B ELISA Kit (Human) : 96 Wells (OKEH01676)
Your Rating
We found other products you might like!