MAP1LC3B Antibody (OAGA05294)

Catalog No: OAGA05294

Size:100 ul

Sample (30 ug of whole cell lysate)
A: U87-MG
15% SDS PAGE
diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Rat brain
12% SDS PAGE
diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human Breast carcinoma, using LC3B antibody at 1:500 dilution.

LC3B antibody [N1C3] detects LC3B protein at autophagosome by immunofluorescent analysis.
Samples: HepG2 cells mock (left) and treated with 3 uM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min.
Green: LC3B protein stained by LC3B antibody [N1C3] diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

LC3B antibody [N1C3] detects LC3B protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4C for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: LC3B antibody [N1C3] dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: FACS, ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Full length human LC3B Recombinant protein.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.8 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: FACS: 1:50-1:200, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: MAP1LC3B
• Gene Full Name: microtubule associated protein 1 light chain 3 beta
• Alias Symbols: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3
• NCBI Gene Id: 81631
• Protein Name: microtubule-associated proteins 1A/1B light chain 3B
• Description of Target: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
• Uniprot ID: Q9GZQ8
• Protein Accession #: NP_073729.1
• Nucleotide Accession #: NM_022818.4