MAOB ELISA Kit (Pig) (OKCA02479)

Catalog No: OKCA02479

Size:96 Wells

Pig MAOB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Pig
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  1:5	97	92-102
  1:10	91	85-98
  1:20	90	88-93
  1:40	100	96-104

• ELISA Kit Principle: Aviva Systems Biology MAOB ELISA Kit (Pig) (OKCA02479) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAOB has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAOB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard MAOB captured in the well.
• ELISA Kit Range: 0.312 - 20 U/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	90	87-92
  EDTA Plasma (n=4)	106	103-110

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • MAOB Microplate: 96 Wells (12 x 8 Well strips)
  • MAOB Lyophilized Standard: 2
  • 100X Biotinylated MAOB Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.078 U/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MAOB
• Alias Symbols: RP1-201D17__B.1, MGC26382, MAO, brain, MAO, platelet, adrenalin oxidase, tyramine oxidase
• NCBI Gene Id: 414909
• Protein Name: Amine oxidase [flavin-containing] B
• Description of Target: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
• Uniprot ID: Q6PLK3
• Protein Accession #: NP_001001864
• Nucleotide Accession #: NM_001001864