MAOB ELISA Kit (Dog) (OKEH08807)

Catalog No: OKEH08807

Size:96T

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	81-90% (n = 20)	88-98% (n = 20)	83-94% (n = 20)	101-111% (n = 20)
  EDTA plasma(n=5)	95-107% (n = 20)	84-96% (n = 20)	102-114% (n = 20)	103-113% (n = 20)
  heparin plasma(n=5)	105-116% (n = 20)	97-109% (n = 20)	82-92% (n = 20)	107-117% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology MAOB ELISA Kit (Dog) (OKEH08807) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAOB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAOB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAOB captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=9.0% (n = 20)
  Mean Inter-assay CV%: <=10.0% (n = 20)
• ELISA Kit Component:
  • MAOB Microplate: 96 Wells (12 x 8 Well strips)
  • MAOB Lyophilized Standard: 2
  • 100X Biotinylated MAOB Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
  Subcellular Location: Mitochondrion outer membrane Single-pass type IV membrane protein Cytoplasmic side
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Dog Amine oxidase [flavin-containing] B
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MAOB
• Gene Full Name: monoamine oxidase B
• Alias Symbols: amine oxidase [flavin-containing] B;MAO-B;monoamine oxidase type B.
• NCBI Gene Id: 403451
• Protein Name: Q7YRB7
• Description of Target: Amine oxidase [flavin-containing] B
• Uniprot ID: https://www.uniprot.org/uniprot/Q7YRB7
• Protein Accession #: NP_001002970.1
• Nucleotide Accession #: NM_001002970.1