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MAOA ELISA Kit (Rat) (OKEH03176)

96 Wells
$529.00
In Stock
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Predicted Species Reactivity:
Rat
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
MAOA
Official Gene Full Name:
monoamine oxidase A
Alias Symbols:
MAO-A, Monoamine oxidase type A
NCBI Gene Id:
29253
Protein Name:
Amine oxidase [flavin-containing] A
Description of Target:
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
Swissprot Id:
P21396
Protein Accession #:
NP_387502.1
Nucleotide Accession #:
NM_033653.1
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.164 ng/mL
Kit Range:
0.312 - 20ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology MAOA ELISA Kit (Rat) (OKEH03176) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Maoa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Maoa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Maoa captured in well.
Kit Component:
ComponentAmount
Anti-Maoa Microplate96 Wells (12 x 8 Well strips)
Maoa Lyophilized Standard2
100X Biotinylated Maoa Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Maoa.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Maoa.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Rat Amine oxidase [flavin-containing] A
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
60 kDa
Protein Size (# AA):
526

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