- Official Gene Full Name:
- monoamine oxidase A
- NCBI Gene Id:
- Alias Symbols:
- 1110061B18Rik, AA407771, MAO-A, MGC27811, Monoamine oxidase type A
- Description of Target:
- Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
- Protein Name:
- Amine oxidase [flavin-containing] A
- Predicted Species Reactivity:
- Sample Type:
- Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
- 0.078 ng/mL
- Kit Range:
- 0.156 - 10 ng/mL
- Kit Reproducibility:
- Mean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
- Kit Duration:
- ~ 3 Hours
- Kit Principle:
- Aviva Systems Biology MAOA ELISA Kit (Mouse) (OKEH03177) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Maoa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Maoa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Maoa captured in well.
- Kit Component:
|Anti-Maoa Microplate||96 Wells (12 x 8 Well strips)|
|Maoa Lyophilized Standard||2|
|100X Biotinylated Maoa Detector Antibody||1 x 120 uL|
|100X Avidin-HRP Conjugate||1 x 120 uL|
|Sample Diluent||1 x 20 mL|
|Detector Antibody Diluent||1 x 12 mL|
|Conjugate Diluent||1 x 12 mL|
|25X Wash Buffer||1 x 30 mL|
|TMB Substrate||1 x 10 mL|
|Stop Solution||1 x 10 mL|
- Kit Recovery:
- Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
- Kit Detection Method:
- Colorimetric, OD450 nm
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express Maoa.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express Maoa.
- Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
- Reconstitution and Storage:
- Store as indicated in product manual.
- This assay recognizes recombinant and natural mouse AOFA. No significant cross-reactivity or interference was observed.
- Assay Info:
- Assay Methodology: Quantitative Sandwich Immunoassay