Mannose Binding Lectin Antibody ELISA Kit (Human) (OKCD01297)

Catalog No: OKCD01297

Size:96WELLS

Human Mannose Binding Lectin ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h, 30min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-97%	97-105%	93-101%	93-101%
  EDTA Plasma (n=5)	86-93%	93-103%	83-104%	95-103%
  Heparin Plasma (n=5)	95-103%	80-103%	79-98%	80-101%

• ELISA Kit Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Mannose Binding Lectin Antibody (Anti-MBL) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Mannose Binding Lectin Antibody (Anti-MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 6.25-400ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-104	101
  EDTA Plasma (n=5)	79-104	99
  Heparin Plasma (n=5)	81-105	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mannose Binding Lectin Antibody (Anti-MBL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mannose Binding Lectin Antibody (Anti-MBL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Mannose Binding Lectin antibody Microplate: 96 Wells (12 x 8 Well strips)
  • Mannose Binding Lectin antibody Lyophilized Standard: 2 x XXX
  • 100X Detector Antibody: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 2.45 ng/mL
• Assay Info: Assay Method: Quantitative Reverse Capture Sandwich ELISA

Target Info

• Gene Symbol: MBL2
• Gene Full Name: mannose binding lectin 2
• Alias Symbols: COLEC1;collectin-1;HSMBPC;mannan-binding lectin;Mannan-binding protein;Mannose-binding lectin;mannose-binding lectin (protein C) 2, soluble (opsonic defect);mannose-binding lectin 2, soluble (opsonic defect);mannose-binding protein C;MBL;MBL2D;MBP;MBP1;MBP-C;MBPD.
• NCBI Gene Id: 4153
• Protein Name: Mannose-binding protein C
• Description of Target: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
• Uniprot ID: P11226
• Nucleotide Accession #: NM_000242.2