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Catalog No: OKSA11290 (Formerly GWB-Q00458)
Size:25T
Price: $419.00
SKU
OKSA11290
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MAGIC RED CATHEPSIN B KIT (OKSA11290)

Datasheets/ManualsPrintable datasheet for MAGIC RED CATHEPSIN B KIT (OKSA11290)
Product Info
Predicted Species ReactivityHuman
Product FormatKit
ApplicationIF, IHC
ELISA Kit ComponentMagic RedTM MR-(RR)2
Hoechst 33342 Stain
Acridine orange (AO)
Reconstitution and Storage2°C to 8°C
SpecificityCATHEPSIN B
Description of TargetCathepsin Magic RedTM kits measure cathepsin B protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product. The Magic RedTM reagent contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe. The Cathepsin Magic RedTM kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits. This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
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