MAGIC RED CATHEPSIN B KIT (OKSA11290)

Catalog No: OKSA11290 (Formerly GWB-Q00458)

Size:25T

Product Info

• Predicted Species Reactivity: Human
• Product Format: Kit
• Application: Immunofluorescence
• ELISA Kit Component: Magic Red^TM MR-(RR)_{2
  Hoechst 33342 Stain
  Acridine orange (AO)}
• Reconstitution and Storage: 2°C to 8°C
• Specificity: CATHEPSIN B

Target Info

• Description of Target: Cathepsin Magic Red^TM kits measure cathepsin B protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product. The Magic Red^TM reagent contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe. The Cathepsin Magic Red^TM kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits. This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.