MAGEB2 Antibody - N-terminal region (OAAB16520)

Catalog No: OAAB16520

Size:400ul

Western blot analysis of MAGEB2(arrow) using rabbit polyclonal MAGEB2 Antibody (Cat.# OAAB16520). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAGEB2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
  Function: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
  Tissue Location: Expressed in testis and placenta, and in a significant fraction of tumors of various histologic types.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MAGEB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-33 amino acids from the N-terminal region of human MAGEB2.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: MAGEB2
• Alias Symbols: DAM6, CT3.2, MAGE-XP-2
• NCBI Gene Id: 4113
• Protein Name: Melanoma-associated antigen B2
• Description of Target: MAGEB2 is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region. It is expressed in testis and placenta, and in a significant fraction of tumors of various histological types. The MAGEB genes are clustered on chromosome Xp22-p21.
• Uniprot ID: O15479
• Protein Accession #: NP_002355.2
• Molecular Weight: 35 kDa