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Catalog No: OCOA17740
Size:20UG
Price: $169.00
SKU
OCOA17740
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MAGEA4 Protein Lysate (OCOA17740)

Datasheets/ManualsPrintable datasheet for MAGEA4 Protein Lysate (OCOA17740)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionMAGEA4 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolMAGEA4
Gene Full NameMAGE family member A4
Alias Symbolscancer/testis antigen 1.4;cancer/testis antigen family 1, member 4;CT1.4;MAGE4;MAGE-4 antigen;MAGE-41;MAGE-41 antigen;MAGE4A;MAGE4B;MAGE-X2;MAGE-X2 antigen;melanoma antigen family A, 4;melanoma antigen family A4;melanoma-associated antigen 4.
NCBI Gene Id4103
Protein NameA0A024RC12, NM_001011549, NP_001011549, P43358
Description of TargetHomo sapiens MAGE family member A4 (MAGEA4), transcript variant 3, mRNA, Melanoma antigen family A, 4, isoform CRA_a, Melanoma-associated antigen 4, melanoma-associated antigen 4 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P43358
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001011549
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001011549
Molecular Weight34.9 kDa
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