MAG ELISA Kit (Human) : 96 Wells (OKEH00439)

Catalog No: OKEH00439

Size:96T

Human MAG ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MAG ELISA Kit (Human) (OKEH00439) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human MAG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human MAG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human MAG captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 69%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.9% (n = 20)
  Mean Inter-assay CV%: < 8.5% (n = 20)
• ELISA Kit Component:
  • Anti-MAG Microplate: 96 Wells (12 x 8 Well strips)
  • MAG Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated MAG: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.036 ng/mL
• Specificity: Natural and recombinant Human Myelin-associated glycoprotein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for MAG ELISA Kit (Human) : 96 Wells (OKEH00439) on CiteAb

Target Info

• Gene Symbol: MAG
• Gene Full Name: myelin associated glycoprotein
• Alias Symbols: GMA;myelin-associated glycoprotein;sialic acid binding Ig-like lectin 4A;sialic acid-binding immunoglobulin-like lectin 4A;SIGLEC4A;SIGLEC-4A;S-MAG;SPG75.
• NCBI Gene Id: 4099
• Protein Name: P20916
• Description of Target: Myelin-associated glycoprotein
• Uniprot ID: https://www.uniprot.org/uniprot/P20916
• Protein Accession #: NP_001186145.1
• Nucleotide Accession #: NM_001199216.1
• Protein Size (# AA): 626
• Molecular Weight: 69 kDa