MAFG Antibody (OAGA04708)

Catalog No: OAGA04708

Size:100 ul

MAFG antibody detects MAFG protein by western blot analysis.
A.50 ug mouse muscle lysate/extract
12% SDS-PAGE
MAFG antibody dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

MAFG antibody detects MAFG protein by western blot analysis.
A.50 ug rat muscle lysate/extract
12% SDS-PAGE
MAFG antibody dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using MAFG antibody at 1:500 dilution.

MAFG antibody detects MAFG protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAFG protein stained by MAFG antibody diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 um.

Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 ug of MAFG antibody .
Western blot analysis was performed using MAFG antibody .
EasyBlot anti-Mouse IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid1XPBS, 20% glycerol (pH 7) with 0.025% Proclin300 added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, IP, WB, CHIP assay, CHIP-seq
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly before prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MAFG. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.53 mg/ml
• Application Info: WB: 1:500~3000
  ICC/IF: 1:100~1000
  IHC-P: 1:100~1000
  IP: 1:100~500
  ChIP assay: Assay dependent
  ChIP-seq: Assay dependent

Target Info

• Gene Symbol: MAFG
• Gene Full Name: MAF bZIP transcription factor G
• Alias Symbols: hMAF
• NCBI Gene Id: 4097
• Protein Name: transcription factor MafG
• Description of Target: Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters. NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).
• Uniprot ID: O15525
• Protein Accession #: NP_002350.1
• Nucleotide Accession #: NM_002359.3
• Molecular Weight: 18 kDa