MAF ELISA Kit (Rat) (OKEH03306)

Catalog No: OKEH03306

Size:96T

Rat MAF ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MAF ELISA Kit (Rat) (OKEH03306) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Maf has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Maf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Maf captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • Anti-Maf Microplate: 96 Wells (12 x 8 Well strips)
  • Maf Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Maf Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Rat Transcription factor Maf
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Maf
• Gene Full Name: MAF bZIP transcription factor
• Alias Symbols: bZip transcription factor c-maf;cMaf;C-Maf;Maf2;proto-oncogene c-maf;transcription factor Maf;transcription factor Maf-2;v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog;V-maf musculoaponeurotic fibrosarcoma oncogene homolog.
• NCBI Gene Id: 54267
• Protein Name: P54844
• Description of Target: Transcription factor Maf
• Uniprot ID: https://www.uniprot.org/uniprot/P54844
• Protein Accession #: NP_062191.1
• Nucleotide Accession #: NM_019318.1
• Protein Size (# AA): 369
• Molecular Weight: 38 kDa