M6PR ELISA Kit (Human) (OKCD00709)

Catalog No: OKCD00709 (Formerly GWB-KBBUA9)

Size:96WELLS

Human M6PR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	95-105%	92-105%	87-96%	90-104%
  EDTA plasma(n=5)	80-105%	88-98%	86-93%	94-102%
  heparin plasma(n=5)	88-95%	90-97%	84-98%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose-6-Phosphate Receptor (M6PR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose-6-Phosphate Receptor (M6PR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose-6-Phosphate Receptor (M6PR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mannose-6-Phosphate Receptor (M6PR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	94-101	97
  EDTA plasma(n=5)	85-99	89
  heparin plasma(n=5)	88-104	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose-6-Phosphate Receptor (M6PR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose-6-Phosphate Receptor (M6PR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-M6PR Microplate: 96 Wells (12 x 8 Well Strips)
  • M6PR Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated M6PR Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.63 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mannose-6-Phosphate Receptor (M6PR). No significant cross-reactivity or interference between Mannose-6-Phosphate Receptor (M6PR) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: M6PR
• Gene Full Name: mannose-6-phosphate receptor, cation dependent
• Alias Symbols: 46 kDa mannose 6-phosphate receptor;46-kDa mannose 6-phosphate receptor;cation-dependent mannose-6-phosphate receptor;CD Man-6-P receptor;CD-M6PR;CD-MPR;MPR 46;MPR46;MPR-46;Mr 46,000 Man6PR;small mannose 6-phosphate receptor;SMPR.
• NCBI Gene Id: 4074
• Protein Name: Cation-dependent mannose-6-phosphate receptor
• Description of Target: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
• Uniprot ID: P20645
• Protein Accession #: NP_002346
• Nucleotide Accession #: NM_002355.3
• Protein Size (# AA): 277