Catalog No: OKCD01196 (Formerly GWB-KBBJU3)
Size:96WELLS
Price: $700.00
SKU
OKCD01196
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Datasheets/ManualsPrintable datasheet for LYZ1 ELISA Kit (Rat) (OKCD01196)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)80-101%78-102%80-95%85-92%
EDTA plasma(n=5)89-97%79-89%80-102%78-105%
heparin plasma(n=5)82-102%95-102%96-104%84-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysozyme (LZM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysozyme (LZM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysozyme (LZM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysozyme (LZM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)81-10390
EDTA plasma(n=5)78-8982
heparin plasma(n=5)83-9288
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysozyme (LZM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysozyme (LZM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Lyz1 Microplate96 Wells (12 x 8 Well Strips)
Lyz1 Lyophilized Standard2 x 5 ng
100X Biotinylated Lyz1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 36 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lysozyme (LZM). No significant cross-reactivity or interference between Lysozyme (LZM) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLYZ1
Gene Full Namelysozyme 1
Alias Symbols1,4-beta-N-acetylmuramidase C.
NCBI Gene Id25211
Protein NameLysozyme C-1
Description of TargetLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. In the intestine they may also have a digestive function.
Uniprot IDP00697
Protein Accession #NP_036903
Nucleotide Accession #NM_012771.3
Protein Size (# AA)148
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