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Catalog No: OKCA00263
Size:96 Wells
Price: $550.00
SKU
OKCA00263
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Lysozyme ELISA Kit (Chicken) : 96 Wells (OKCA00263)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKCA00263
Product Info
Predicted Species ReactivityChicken
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:2084%81-93%
Serum (n=4)1:4085%82-99%
Serum (n=4)1:8090%85-103%
Serum (n=4)1:16094%87-105%
Sample Type 3
Sample TypeDilutionAverage %Range %
Egg white (n=4)1:10,00086%80-95%
Egg white (n=4)1:20,00089%84-100%
Egg white (n=4)1:40,00093%86-107%
Egg white (n=4)1:80,00096%90-110%
ELISA Kit PrincipleAviva Systems Biology Lysozyme ELISA Kit (Chicken) (OKCA00263) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Chicken Lysozyme antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Lysozyme and incubated. The Lysozyme found in the sample or standards competes with the biotinylated Lysozyme for limited binding sites on the immobilized anti-Chicken Lysozyme antibody. Excess unbound biotinylated Lysozyme and sample or standard Lysozyme is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Lysozyme captured in the well and inversely proportional to the amount of Lysozyme which was contained in the sample or standard.
ELISA Kit Range15.625 ng/mL - 4,000 ng/mL
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (ng/mL)249.61247.756250.857248.99246.53254.012
SD0.0260.0290.0310.0390.0410.046
CV%3.6214.0224.335.4245.6716.471
ELISA Kit Component
ComponentAmount
Anti-Lysozyme Microplate96 Well (12 x 8 Well Strips)
Lysozyme Standard (Lyophilized)2
100X Lysozyme-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 10 mL
Conjugate Diluent1 x 20 mL
Sample Diluent2 x 20 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Egg white
Sensitivity14.876 ng/ml
SpecificityThis assay has high sensitivity and excellent specificity for detection of chicken
lysozyme. No significant cross-reactivity or interference between chicken
lysozyme and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene SymbolLysozyme
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