Lysis Buffer: Membrane Proteins (OLCD00001)

Catalog No: OLCD00001

Product Info

• Product Format: Liquid 10⁷ cells/50 mg tissues by 1 mL lysis buffer
• Reconstitution and Storage: Store at 4°C for frequent use. Stored at -20°C for one year. Avoid repeated freeze-thaw cycles. In order to prevent protein degradation, it is recommended to add 1-2 mM PMSF into the lysis buffer before usage. Lysis process should be performed on ice.
  If salt precipitation occurs after long-term storage, set lysis buffer at 4°C, but it will dissolve after it's placed at room temperature. This phenomenon is normal and would not affect the result of the experiment.
• Application Info: It's applicable on most experiments, such as SDS-PAGE, Western Blotting (WB), Immunoprecipitation (IP), Co-IP, ELISA, etc. Applications include membrane proteins, including surface proteins and receptors.
  Lysis buffer should be chosen according to the characteristics of samples and experimental purposes. Optimal experiment condition should be determined by end-user through pilot experiment.

Target Info

• Description of Target: Lysis buffer is used to lyse cells under non-denaturing buffer conditions. It consists of surfactant, a protease inhibitor, etc. It is mainly used for protein extraction from cells and tissues. This product is optimized from traditional lysis buffer. It's suitable for lysis of membrane proteins, including surface proteins and receptotrs. which will help the release of proteins and do good for later extraciton, ELISA, Western Blot, etc.