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Lysis Buffer: Membrane Proteins (OLCD00001)

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Description of Target:
Lysis buffer is used to lyse cells under non-denaturing buffer conditions. It consists of surfactant, a protease inhibitor, etc. It is mainly used for protein extraction from cells and tissues.
Product Format:
Liquid
Application:
ELISA
Application Info:
Membrane proteins including surface proteins, receptors
Additional Information:
Usage: 10^7 cells/50mg tissues by 1 ml lysis buffer.
:::
Important Notes:
Avoid freeze-thaw cycles, aliquot when receiving.
Lysis process should be performed on ice.
If salt precipitation occurs after long-term storage, set lysis buffer at room temperature to dissolve precipitate.
Lysis buffer should be chosen according to the characteristics of samples and experimental purposes.
Optimal experiment condition should be determined by end-user through pilot experiment.
Contains sodium azide which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.
Reconstitution and Storage:
Store at 4°C for frequent use. Stored at -20°C for one year. Avoid repeated freeze-thaw cycles. In order to avoid protein degradation, it is recommended to add 1-2mM PMSF into the Lysis Buffer before usage.
Datasheets/Manuals:
Printable datasheet for OLCD00001
Protocol:
Tips Information:

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