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Datasheets/Manuals | Printable datasheet for LY9 Antibody (OAEE00154) (OAEE00154) |
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Predicted Species Reactivity | Human |
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Product Format | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Clonality | Monoclonal |
Clone | HLy9.25 |
Isotype | IgG1 |
Host | Mouse |
Application | Flow cytometry|Immunocytochemistry|Immunoprecipitation|Western blot |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | CD299-transfected 300.19 pre-B cell line |
Purification | Purified |
Concentration | 1 mg/ml |
Specificity | The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells. |
Formulation | Purified |
Purity | > 95% (by SDS-PAGE) |
Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
Reference | Martín M, Del Valle JM, Saborit I, Engel P: Identification of Grb2 as a novel binding partner of the signaling lymphocytic activation molecule-associated protein binding receptor CD229. J Immunol. 2005 May 15;174(10):5977-86. Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse. J Immunol. 2005 Jun 1;174(11):7033-42. de la Fuente MA, Tovar V, Villamor N, Zapater N, Pizcueta P, Campo E, Bosch J, Engel P: Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9. Blood. 2001 Jun 1;97(11):3513-20. Sintes J, Romero X, Marin P, Terhorst C, Engel P: Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells. Exp Hematol. 2008 Sep;36(9):1199-204. Romero X, Benítez D, March S, Vilella R, Miralpeix M, Engel P: Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue Antigens. 2004 Aug;64(2):132-44. Bund D, Mayr C, Kofler DM, Hallek M, Wendtner CM: Human Ly9 (CD229) as novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (B-CLL) recognized by autologous CD8+ T cells. Exp Hematol. 2006 Jul;34(7):860-9. Del Valle JM, Engel P, Martín M: The cell surface expression of SAP-binding receptor CD229 is regulated via its interaction with clathrin-associated adaptor complex 2 (AP-2). J Biol Chem. 2003 May 9;278(19):17430-7. |
Gene Symbol | LY9 |
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Gene Full Name | lymphocyte antigen 9 |
Alias Symbols | CD229;cell surface molecule Ly-9;hly9;Lymphocyte antigen 9;mLY9;signaling lymphocytic activation molecule 3;SLAM family member 3;SLAMF3;T-lymphocyte surface antigen Ly-9. |
NCBI Gene Id | 4063 |
Protein Name | T-lymphocyte surface antigen Ly-9 |
Description of Target | Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A (PubMed:22184727). Promotes recruitment of RORC to the IL-17 promoter (PubMed:22989874). May be involved in the maintenance of peripheral cell tolerance by serving as a negative regulator of the immune response. May disable autoantibody responses and inhibit IFN-gamma secretion by CD4(+) T-cells. May negatively regulate the size of thymic innate CD8(+) T-cells and the development of invariant natural killer T (iNKT) cells (By similarity). |
Uniprot ID | Q9HBG7 |
Protein Accession # | NP_001028839.1; NP_001248385.1; NP_002339.2 |
Nucleotide Accession # | NM_001033667.2; NM_001261456.1; NM_002348.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "LY9 Antibody (OAEE00154)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "LY9 Antibody (OAEE00154)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "LY9 Antibody (OAEE00154)" provided in?
This item is provided in "Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "LY9 Antibody (OAEE00154)"?
This target may also be called "CD229;cell surface molecule Ly-9;hly9;Lymphocyte antigen 9;mLY9;signaling lymphocytic activation molecule 3;SLAM family member 3;SLAMF3;T-lymphocyte surface antigen Ly-9." in publications.
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What is the shipping cost for "LY9 Antibody (OAEE00154)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "LY9 Antibody (OAEE00154)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "LY9 Antibody (OAEE00154)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "LY9 Antibody (OAEE00154)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "LY9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "LY9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "LY9"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "LY9"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "LY9"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "LY9"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.