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Catalog No: OKEH04256
Size:96T
Price: $525.00
SKU
OKEH04256
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LY6G6F ELISA Kit (Human) (OKEH04256) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LY6G6F has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LY6G6F is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LY6G6F captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.8% (n = 20)
Mean Inter-assay CV%: <= 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
LY6G6F Microplate96 Wells (12 x 8 Well Strips)
LY6G6F Standard2 x 2 ng
Sample Diluent1 x 20 mL
LY6G6F Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.089 ng/mL
SpecificityNatural and recombinant Human Lymphocyte antigen 6 complex locus protein G6f
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLY6G6F
Gene Full Namelymphocyte antigen 6 family member G6F
Alias SymbolsC6orf21;G6f;LY6G6;LY6G6D;lymphocyte antigen 6 complex locus protein G6f;lymphocyte antigen 6 complex, locus G6D;lymphocyte antigen 6 complex, locus G6F;NG32.
NCBI Gene Id259215
Protein NameQ5SQ64
Description of TargetLymphocyte antigen 6 complex locus protein G6f
Uniprot IDhttps://www.uniprot.org/uniprot/Q5SQ64
Protein Accession #NP_001003693.1
Nucleotide Accession #NM_001003693.1
Protein Size (# AA)297
Molecular Weight32 kDa
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