Luteinizing Hormone ELISA Kit (Sheep) : 96 Wells (OKCA00331)

Catalog No: OKCA00331

Size:96 Wells

Product Info

• Predicted Species Reactivity: Sheep
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	90%	85-104%
  Serum (n=4)	1:2	95%	90-99%
  Serum (n=4)	1:4	101%	98-111%
  Serum (n=4)	1:8	85%	80-97%

• ELISA Kit Principle: Aviva Systems Biology Luteinizing Hormone ELISA Kit (Sheep) (OKCA00331) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Sheep monoclonal antibody specific for Sheep Luteinizing Hormone has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Sheep Luteinizing Hormone is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sheep Luteinizing Hormone captured in well.
• ELISA Kit Range: 2 mIU/mL - 75 mIU/mL
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (mIU/mL)	19.918	19.671	20.387	20.915	19.859	19.407
  SD	0.039	0.041	0.037	0.048	0.047	0.046
  CV%	5.891	6.331	5.553	7.012	7.231	7.189

• ELISA Kit Component:
  • Anti-Luteinizing Hormone Microplate: 96 Well (12 x 8 Well Strips)
  • Luteinizing Hormone Standard (Lyophilized): 6
  • HRP-Conjugate: 1 x 6 mL
  • 20X Wash Buffer: 1 x 15 mL
  • Substrate A: 1 x 7 mL
  • Substrate B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: 0.5 mIU/ml
• Specificity: This assay has high sensitivity and excellent specificity for detection of sheep LH.
  No significant cross-reactivity or interference between sheep LH and analogues
  was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Luteinizing Hormone