Catalog No: OKCA00399
Size:96 Wells
Price: $754.00
SKU
OKCA00399
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Lupus Anticoagulant Antibody (IgG) (Human) ELISA Kit (OKCA00399)
Predicted Species Reactivity | Human | ||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Lupus Anticoagulant Antibody (IgG) (Human) ELISA Kit (OKCA00399) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Lupus Anticoagulant antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanIgG is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Lupus Anticoagulant Antibody (IgG) captured in well. | ||||||||||||||||||||
ELISA Kit Reproducibility | Three different samples of known Lupus Anticoagulant Antibody (IgG) concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each. Intra-Assay CV: <15% Inter-Assay CV: <15% | ||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
Sample Type | Serum | ||||||||||||||||||||
Assay Info | Assay Methodology: Qualitative Reverse Capture ELISA | ||||||||||||||||||||
Protocol Information |
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Protein Name | Lupus Anticoagulant Antibody (IgG) |
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Description of Target | Lupus Anticoagulant Antibody (IgG) |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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